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Very useful video. Can you tell us what software you used to analyze the data? There has been no answer to the previous question. Thanks for your attention.

Good I don’t have to wonder if I’m getting hiv or drugged never know whats in your goodie bag

hello :) is it possible to damage the antibodies by vortexing them after dilution ?

how long can i storage the antibody-coated elisa plate in the refrigerator?

what's the difference between raw material and starting material? Are starting material considered as one kind of raw material, but just more critical?

cost?

Whomuch instrument cost test Manu

Is it possible to aliquot the reconstituted standard and store it at -80°C? If so, for how long?

Saved my morning, thank you

How long does it take to do the entire workflow?

Hello mam you have provide stem cell therapy Neurogenic bladder

Is it mandatory to use controls with each run?

Wonderful talk!!!

What is an IP?

5:40

Hi. Really nice and useful video. Can you please tell which software you used for data analysis? Thank you.

Great lecture

wow, chymotrypsin-like cleavage (beta5) might be most important for longevity 34:37 "key lysine residue" at 27:00?

very helpful

How does this get into the cell?

Thanks for this great lecture! It includes so many insights, facts and pitfalls that are so easy to overlook.

Very nice video. Sorry to ask, is it necessary to have the handheld magnetic washer?

9:30 start of presentation 13:20 ubiquitin-proteasome pathway summary slide - ATP required 15:22 *antigen presentation* - 1% of product peptides are taken up by ER and displayed in cell surface by MHC class I molecules - proteasome allows immune system to screen intracellular space for abnormal proteins (pathogen or cancer) 23:16 multiple ATP dependent steps in proteasome function 38:00 Bortezmib - proteasome inhibitor, inhibits NF-kB, does not cross BBB, induces apoptosis 40:33 Endoplasmic-reticulum-associated protein degradation () pathway is unuslaully activated in multiple myeloma cells ; proteasome inhibition results in increased ER stress and triggers UPR, further UPR activation triggers apoptosis 42:30 proteasome inhibitors are not more toxic since they mainly block chymotrypsin-like site & only partially inhibit protein degradation ; since myeloma cells have to break down lots of immunoglobins they become more stressed than other cells as a result 43:27 cells can compensate for proteasome inhibition thru Nrf1 activation (more proteasome) and increase in autophagy / lysosomal degradation 44:35 cAMP phosphorylation of 26S proteasome enhances degradation of misfolded proteins 45:50 cAMP dependent regulation of protein phosphorylation ; rolipram is PDE4 inhibitor 47:39 cAMP activation for treatment of neurodegenerative diseases 49:06 rolipram for taupathology ; rolipram increased proteasome activity and reduced tau accumulation in mouse model 50:36 summary slide - endogenous regulation (ubiquitination, DUBs) ; exogenous activation - phosphorylation of proteasome 52:13 Q&A

very good video

Thank u !! I have an exam tomorrow of this and It helped me a lot

What is the purpose of phosphate buffered saline?

thanks for this video

But what isn't addressed here and most commonly anywhere else is how long do we need to fast and at whicj juncture - is it at 16 hrs without food or 24 or 36? Then how long do we stay without food for this to be optimally beneficial. Tell.us the whole story. Break it down to a simplistic practical application please.

On your investor web page you titled this video "Epic Tools for Epic Science" (investors.bio-techne.com/). That is a lot more compelling than "A Reputation of Quality: The Bio-Techne Brand Story". Own it.

Hey. Excellent webinar!!. I have one question, in normoxia, how many passages can we culture the MSCs?.. Also, we are facing difficulty in trypsinization of MSCs. any suggestions??

I love your slides. Very comprehensable explanations too 5|5

Does this work with all cells lines?

Nice video

Good work!

23:00 ,2nd lecturE obecity impact on stomach & cancer

Is it in the competition with Organs on Chip technology ...this is more accurate and has more reliable results ...

I am not from the scientific community. However I have been practicing “ starvation” to activate autophagy the last 10 years without realizing it. I take just 1 meal daily. Dinner. I skip breakfast and lunch. I do not eat anything after dinner. No special diet. What is most interesting to me is the realization that the ancients have knew about autophagy all this while. Muslims fast. The gurus of India and the lama in Tibet and Nepal fast on a regular basis. They know the benefits of autophagy. The only difference is the do not understand mechanism of it until 20th/21st century with the ground breaking discovery of Dr Yoshinori Ohsumi. It is also very interesting to find out that certain existing drugs may help in activating autophagy. Metformin for example has is commonly prescribed for diabetes.

Go to 3:41 to skip the tedious introduction

So people with ms should avoid dairy?

thank you for uploading this, I am beginning my honours project this year which is related to ARMeD destruction of endogenous proteins. this video on SUMO has helped me get my head around the basics.